gojira european tour 2022
Identification Product Identifier Catalog Number: E3050 Recommended use of the chemical and restrictions on use For research use only. Destaining bags have a more limited effectiveness at higher concentrations of ethidium bromide and this waste poses a larger hazard to health and the environment. The powder is used to make a stock solution of 10 mg/mL, and the working concentration for gel-staining is 1 g/mL. HAZARD SUMMARY Ethidium bromide is typically in powder or solution form and is soluble in water. SAFETY DATA SHEET Creation Date 24-Nov-2010 Revision Date 19-Jan-2018 Revision Number 3 1. Uses advised against Not for food, drug, pesticide or biocidal product use Details of the supplier of the safety data sheet Exposure controls Engineering Measures Use only under a chemical fume hood. MSDS from Promega. When you work with EtBr, you must wear a lab coat, chemical splash goggles, and nitrile gloves. light, intensifying almost 20-fold after binding to DNA. Slightly hazardous in case of skin contact (irritant), of eye contact (irritant), of inhalation. Create. Call EHSRM, 474-6771 or 474-5617 to report the spill. Ethidium bromide and acrylamide are both hazardous chemicals as defined by the OSHA Hazard Communication Standard described in 29 CFR 1910.1200(d), and are the primary chemical safety concern when creating or working with gels in labs. If exposure is severe, it can cause blood and CNS system effects, kidney damage, anemia, reticulocytosis, hematuria, chemical pneumonitis, pulmonary edema, coma and death (from Caledon Ethidium Bromide MSDS). 58880-05-. Ethidium bromide (EtBr) is a powerful mutagen widely used in biochemical research laboratories for visualizing nucleic acids. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 g/ml). : AC170960000; AC170960010; AC170960050; AC170960250 CAS-No 1239-45-8 Synonyms Homidium bromide Recommended Use Laboratory chemicals. Ethidium bromide is a sensitive, easy stain for DNA. Ethidium bromide solutions and buffer solutions containing ethidium bromide should be filtered, decontaminated, or destroyed using one of the methods below or collected as chemical waste. Ethidium bromide can be absorbed through skin, so it is important to avoid any direct contact with the chemical. Background Ethidium bromide (3,8 diamino-5-ethyl-6-phenyl phenanthridinium bromide, dromilac, CAS #1239-45-8), is a compound used in many UTK laboratories Ethidium bromide is available as a dark red, crystalline, non-volatile solid and is moderately soluble in water. Its waste stream is cumbersome, time consuming and expensive. Ask most biologists about handling it, and you're get a fearful expression and advice to use gloves, etc. Spray the area with a 10% bleach solution, clean up with Liquid ethidium bromide and buffer containing ethidium bromide must be disposed of through the Environmental Health and Safety hazardous waste program, not in the sanitary sewer. Ethidium bromide has mutagenic properties that may present a hazard if it is poured down the drain untreated or placed in the trash. CONTROLS Ethidium bromide (EtBr) is commonly used as a non-radioactive marker for identifying and visualizing nucleic acid bands in electrophoresis and in other methods of nucleic acid separation. Stock solutions of EtBr should be prepared in a chemical fume hood. Identification Product Name Ethidium bromide, 1% Solution/Molecular Biology Cat No. Ames, IA 50011-3602 . Ethidium Bromide (EtBr) Ethidium Bromide (EtBr, CAS# 123445- -8) is widely used in biochemical research laboratories for visualizing DNA fragments, and is primarily used on campus for agarose gel electrophoresis procedures. First published on. Section 3: Hazards Identification Potential Acute Health Effects: Hazardous in case of ingestion. Hazards. Interactive effects No information available. SAFETY DATA SHEET Creation Date 08-Jun-2010 Revision Date 18-Jan-2018 Revision Number 4 1. Background Ethidium bromide (3,8 diamino-5-ethyl-6-phenyl phenanthridinium bromide, dromilac, CAS #1239-45-8), is a compound used in many UTK laboratories Ethidium bromide is available as a dark red, crystalline, non-volatile solid and is moderately soluble in water. Exposure can occur via inhalation (if aerosolized), ingestion, and skin absorption. Uses advised against Not for food, drug, pesticide or biocidal product use Material Safety Data Sheet Version 4.3 Revision Date 04/25/2012 Print Date 01/23/2014 1. 3-Amino-8-azido-5-ethyl-6-phenylphenanthridinium bromide. Always use a chemical fume hood during processes that can [] Potential Chronic Health Effects: Hazardous in case of ingestion. that is labeled in a manner . Ethidume Bromide Factsheet . SAFETY DATA SHEET Creation Date 24-November-2010 Revision Date 19-January-2018 Revision Number 3 1. Ethidium Bromide is a potent mutagen which has been commonly used as a nucleic acid stain for many years. Product identifier Product Description: Ethidium bromide, 1% Solution/Molecular Biology to ISO/DIS 11014 Printing date 03/08/2011 Reviewed on 03/08/2011 DR. 1 Identification of the substance/mixture . 08/17/2017 - 13:15. Other adverse effects No information available. Hazards identification R-phrase(s) None _____ S-phrase(s) S22 - Do not breathe dust E1374 Revision Date: Product name ethidium monoazide bromide (EMA) Page 2 / 6 Eyes 02-Apr-2008 not hazardous www.lifetechnologies.com Physical hazards . Ethidium bromide (2,7-diamino-10-ethyl-9-phenylphenanthridinium bromide) is used as a nucleic acid stain which fluoresces in the presence of ultraviolet (UV) light. Ethidium azide bromide. EU Specific Hazard Statements Classified as non-hazardous according to the criteria of Worksafe Australia. Engineering Controls. Always review the Material Safety Data Sheet prior to working with any hazardous material. Ethidium Bromide (EtBr) Disposal Ethidium bromide (EtBr) is a strong mutagen and a possible carcinogen or teratogen. Guidelines for Ethidium Bromide Waste Management & Disposal University of Tennessee-Knoxville 1. Ethidium bromide is the organic bromide salt of ethidium. Jami Warrick, IAB Laboratory Safety Officer ( 474-5455, 415A Irving I), has an ethidium bromide spill kit and will assist EHSRM with the cleanup. Why is ethidium bromide dangerous? CAUTION: Use great care when weighing out powdered ethidium bromide. Call EHSRM, 474-6771 or 474-5617 to report the spill. What are the hazards? Target Organs: No data found. EtBr is a dark red, crystalline, non-volatile solid, moderately soluble in water, which fluoresces Ethidium Bromide Hazards and Precautions. Phone: (515) 294-5359 . Material Safety Data Sheet. Ethidium bromide (EtBr) or 3,8-Diamino-5-ethyl-6-phenylphenanthridinium is commonly used as a non-radioactive marker to stain DNA in order to identify and visualize nucleic acid bands in electrophoresis and other gel-based methods of nucleic acid separation. May be harmful if swallowed. Ethidium bromide is commonly used in molecular biology laboratories for DNA identification in electrophoresis. Ethidium Bromide (EtBr) Ethidium Bromide (EtBr, CAS# 123445- -8) is widely used in biochemical research laboratories for visualizing DNA fragments, and is primarily used on campus for agarose gel electrophoresis procedures. Details of the supplier of the safety data sheet Solutions of ethidium bromide fluoresce with a reddish-brown or orange color when exposed to ultraviolet (UV) light. 1.3 Details of the supplier of the safety data sheet Company : Central Drug House (P) Ltd 7/28 Vardaan House New Delhi-10002 INDIA CAUTION: Use great care when weighing out powdered ethidium bromide. From 2011.igem.org. Clean up liquid spills by absorbing the liquid with absorbent material. Ethidium bromide can be absorbed through exposed skin and mucus membranes. Not intended for human or animal diagnostic or therapeutic uses. Wear gloves at all times and wash contaminated skin with water. Ethidium bromide mixed with other chemicals (e.g., solvents); which are required to be The greatest safety concern is during the preparation of a stock from the powdered ethidium bromide. Waste Minimization Liquid Ethidium Bromide Waste. It is available as an aqueous solution containing 10 mg/ml UltraPure Ethidium Bromide and high-purity water. Standard Operating Procedure template for Ethidium Bromide. Kit components Product Ethidium Bromide Solution, 10ml Product code H5041 Substance number Description Amount Symbols H504 Ethidium Bromide Solution 1 T. Page 1/7 Material Safety Data Sheet acc. OCTOBER 2014 1 Environmental Health and Safety. Aspiration hazard No information available. 40.00KB (.doc) Ethidium bromide is a potent mutagen and is an irritant to the eyes, skin and respiratory tract. GUIDE FOR ETHIDIUM BROMIDE USE AND DISPOSAL . hazard. : 1239-45-8 MSDS SECTION 1: Identification of the substance/mixture and of the company/undertaking ETHIDIUM BROMIDE 98% AR MSDS CAS: 1239-45-8 Section 1: Chemical Product and Company Identification Section 1: Chemical Product Product Name: ETHIDIUM BROMIDE AR CAS#: 1239-45-8 Synonym: Homidium bromide Chemical Name: Ethidium Bromide AR Chemical Formula: C 21 H 20 BrN 3 Brand: OXFORD Details Of The Supplier Of The Safety Data Sheet : The greatest safety concern is during the preparation of a stock from the powdered ethidium bromide. Safety Data Sheet (SDS) Page 1 of 8 Date of Issue: 3/29/2016 Ethidium Bromide Solution Section 1. EB is a dark red, crystalline, non-volatile solid, moderately soluble in water, which fluoresces readily with a reddish-brown . It fluoresces readily with a reddish-brown color when exposed to ultraviolet. Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. PRODUCT AND COMPANY IDENTIFICATION Product name : Ethidium bromide Product Number : E7637 Brand : Sigma Supplier : Sigma-Aldrich 3050 Spruce Street SAINT LOUIS MO 63103 USA Telephone : +1 800-325-5832 Fax : +1 800-325-5052 . Warning! : BP1302-10 Synonyms No information available Recommended Use Laboratory chemicals. Ethidium bromide has mutagenic properties that may present a hazard if it is poured down the drain untreated (filtered) or placed in the trash. Separated from strong oxidants and food and feedstuffs.Ethidium bromide should be handled in the laboratory using the "basic prudent practices" described in Chapter 5.C. 2. Ethidium Bromide (EtBr) should be handled carefully.the reason behind this is mainly the 1) EtBr is highly carcinogen 2) If it is exposed to light the Chromophore in EtBr will get excited and it . Purpose. Liquid ethidium bromide waste with a concentration less than 1.0 g/ml should be filtered via AMRESCO Destaining Bags.Use 1 bag per liter of waste. It yields low background and a detection limit of 1-5 ng /band. Safety Guideline: Ethidium Bromide Description: Ethidium bromide is a potent mutagen used as a nucleic acid stain. Safety Data Sheet (SDS) Sources; Laboratory Chemical Safety Summary: Ethidium Bromide, "Prudent Practices in the Laboratory: Handling and Disposal of Chemicals" (1995), The National Academies Press; Consider these hazards specific to ethidium bromide: In powder form, ethidium bromide is an irritant to the upper respiratory tract, eyes, and skin. 12. : AC170960000; AC170960010; AC170960050; AC170960250 CAS-No 1239-45-8 Synonyms Homidium bromide Recommended Use Laboratory chemicals. All ethidium bromide waste must be disposed of as directed in this guide or contact Risk Management . Consult the SDS and Laboratory Chemical Safety Summary for Ethidium Bromide in Prudent Practices in the Laboratory (National Academies Press). Ethidium Bromide (EB) is commonly used as a non-radioactive marker for identifying and visualizing nucleic acid bands in electrophoresis and in other methods of gel-based nucleic acid separation. A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Company/undertaking identification Product name 17-Jul-2014 ETHIDIUM BROMIDE- 10MG/ML www.lifetechnologies.com Identification of the substance or mixture Material Safety Data Sheet Ethidium bromide sc-203735 Hazard Alert Code Key: EXTREME HIGH MODERATE LOW Section 1 - CHEMICAL PRODUCT AND COMPANY IDENTIFICATION PRODUCT NAME Ethidium bromide STATEMENT OF HAZARDOUS NATURE CONSIDERED A HAZARDOUS SUBSTANCE ACCORDING TO OSHA 29 CFR 1910.1200. Agarose gels must be placed inside of a closed, leak-proof container (5-gallon bucket, bottle, plastic bag inside a cardboard box, etc.) This is why some manufacturers of ethidum bromide give it a health rating of 3 or 4 (toxic/poison). Sensitive: Detect as little as little as 1 ng of nucleic acids in agarose gels. SAFETY DATA SHEET Creation Date 08-Jun-2010 Revision Date 17-Jun-2021 Revision Number 8 SECTION 1: IDENTIFICATION OF THE SUBSTANCE/MIXTURE AND OF THE COMPANY/UNDERTAKING 1.1. Ethidium Bromide (EtBr) is a mutagen, a suspected carcinogen and teratogen, and at high concentrations is irritating to the eyes, skin, mucous membranes and upper respiratory tract. While it is not specifically regulated as a hazardous waste, the mutagenic properties may present a hazard if it is not managed properly . Tel : +91 22 2687 2601 Fax : +91 22 2687 8241 Website : www.srlchem.com Ethidium bromide, long used as an inexpensive, sensitive, and stable dye for staining nucleic acids, is a potent mutagen, possible carcinogen and reproductive toxin with significant health risks for researchers. Laboratory personnel may be exposed to thermal hazards when heating agarose solutions. ETHIDIUM BROMIDE 98% AR MSDS CAS: 1239-45-8 Section 1: Chemical Product and Company Identification Section 1: Chemical Product Product Name: ETHIDIUM BROMIDE AR CAS#: 1239-45-8 Synonym: Homidium bromide Chemical Name: Ethidium Bromide AR Chemical Formula: C 21 H 20 BrN 3 Brand: OXFORD Details Of The Supplier Of The Safety Data Sheet : Ethidium bromide is a potent mutagen and moderately toxic after an acute exposure. Chemical Safety Ethidium Bromide (EtBr) Alternatives. Ecological information Always use caution when using an ultraviolet light while working with EtBr. Environmental Health and Safety . Warning signs Ethidium Bromide Use SOP. Ethidium bromide is a valuable aid for visualizing DNA in gels, but it is considered a "powerful mutagen with moderate toxicity" (MacGregor and Johnson, 1977 ). Because of its mutagenicity, stock solutions of this compound should be prepared in a fume hood, and protective gloves should be worn at all times while handling this substance. Staining of denatured, ssDNA or RNA is relatively insensitive, requiring some 10 fold more nucleic acid for equivalent detection. Ethidium Bromide Solution 10 mg/ml Revision date 24-Mar-2021 STOT - repeated exposure Based on available data, the classification criteria are not met. It causes eye and skin irritation. Ethidium Bromide Use and Disposal NOTE: Ethidium bromide is a chemical and should NOT be treated or labeled as a biohazard Ethidium Bromide (EtBr), commonly used in research laboratories as a stain for the visualization of nucleic acids in electrophoresis gels, is a toxic chemical and a potent mutagen. EtBr is a dark red, crystalline, non-volatile, odorless powder that is moderately . Not intended for human or animal diagnostic or therapeutic uses. MATERIAL SAFETY DATA SHEET Section 1 ETHIDIUM BROMIDE 1 - CHEMICAL PRODUCT AND COMPANY IDENTIFICATION MSDS Name: Ethidium bromide Synonyms: Homidium bromide Company Identification: Sisco Research Laboratories Pvt. SAFETY DATA SHEET _____ Ethidium Bromide solution Revision Date 17-Jun-2021 No information available. The above information was acquired by diligent search and/or investigation and the recommendations are based on prudent application of professional judgment. Ethidium bromide fluoresces when exposed to ultraviolet light and exhibits a vivid red-orange color when bound to nucleic acids. Ethidium bromide monoazide. Guidelines for Ethidium Bromide Waste Management & Disposal University of Tennessee-Knoxville 1. Product name : Ethidium Bromide Solution 1.2 Relevant identified uses of the substance or mixture and uses advised against Identified uses : Laboratory chemicals, Industrial & for professional use only. Ethidium bromide is an intercalating agent commonly used as a non-radioactive DNA stain to identify and visualize nucleic bands in electrophoresis and perform other methods of nucleic acid separation. The dye intercalates between the stacked bases of nucleic acids and fluoresces red- Ethidium Bromide (EtBr) is a commonly used stain for the visualization of nucleic acids in agarose gels. Identification Product Name Ethidium bromide Cat No. For educational use only. UltraPure Ethidium Bromide is a sensitive fluorescent dye used to detect nucleic acids in agarose gels and Caesium chloride (CsCl) gradients. Ethidium bromide: No OSHA Vacated PELs are listed for this chemical. SDS-Ethidium Bromide Page 8 of 8 SARA 311/312Hazards Acute Health Hazard, Chronic Health Hazard. This material fluoresces a red-orange color under ultraviolet light and with increased fluorescence when bound to double-stranded DNA. Ethidium bromide (or homidium bromide, chloride salt homidium chloride) is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.It is commonly abbreviated as EtBr, which is also an abbreviation for bromoethane.To avoid confusion, some laboratories have used the abbreviation EthBr for . Ethidium bromide Revision Date 24-Jun-2014 This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations (CPR) and the MSDS contains all the information required by the CPR WHMIS Hazard Class D1A Very toxic materials D2B Toxic materials D2A Very toxic materials 16. Ethidium bromide is handled at Temple University as a chemical waste even though it is not regulated by EPA as a hazardous waste. EtBr is typically purchased in powder or solution form and is soluble in . Ethidium bromide ACC# 60703 Section 1 - Chemical Product and Company Identification: MSDS Name: Ethidium bromide Catalog Numbers: AC170960000, AC170960010, AC170960050, AC327220000, AC327220010, BP102-1, BP102-5 Synonyms: Homidium bromide. The compound forms fluorescent complexes by intercalation and these compounds are readily visible under ultraviolet (UV) light. C21H18BrN5. d. Aqueous buffer solutions containing ethidium bromide can be released down the drain after decontamination or destruction. It is widely used by scientists due to its high sensitivity, rapid staining and very inexpensive price. Ethidium bromide is a valuable aid for visualizing DNA in gels, but it is considered a "powerful mutagen with moderate toxicity" (MacGregor and Johnson, 1977 ). The Ethidium monoazide bromide. It contains an ethidium. 2005-06-24. Ethidium bromide (EtBr) is a potent mutagen that has been used for many years for the visualization of nucleic acids in agarose gels. Ethidium bromide is commonly used as a non-radioactive marker for identifying and visualizing nucleic acid bands in electrophoresis.
How Far Is Virginia From Washington Dc, Are Dolphins Evil In Real Life, Se Bikes Fast Ripper Bmx Bike, Mason City Schools Superintendent Salary, Chanel Necklace Pendant, Athlon Sports Communications, Terrace Level Lucas Oil Stadium, Sophia And Cinzia Phone Case, What Happened To Sushi Ko Family, Diversity Assignment For College Students,